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a is definitely the acid dissociation regular. pH-price profiles that showed the loss of catalytic exercise at high and low values of pH were suit to equation 4, exactly where K
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a worth is a bit larger for Cd-NagA than for Zn-NagA, as envisioned from your tendency of zinc to lessen the p
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For that thio-amide substrates, the Cd-enzyme was the best enzyme as well as the Zn-enzyme was poorer. The catalytic constants for your Zn- and Cd-substituted sorts of NagA assist the summary which the carbonyl group of your substrate is polarized by a direct interaction with the single divalent cation certain to the Mβ-position while in the Lively website.